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mouse ckit isolation kit miltenyi biotec  (Miltenyi Biotec)


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    Miltenyi Biotec mouse ckit isolation kit miltenyi biotec
    Mouse Ckit Isolation Kit Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd117+microbeads/CD117+MicroBeads%2C+mouse+-+lyophilized/pm42397745-177-107-111
    Average 96 stars, based on 80 article reviews
    mouse ckit isolation kit miltenyi biotec - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: RPS19 and RPL5 haploinsufficient models reveal divergent ribosomal subunit controls of fetal hematopoiesis.
    Article Snippet: The following antibodies were used: CD4-BV605 (Biolegend, #100451, 1:200); CD8a-BV6-5 (Biolegend, #100744, 1:200); Ter119-BV605 (Biolegend, #116239, 1:200); CD45R/B220-BV605 (Biolegend, #103224, 1:200); Ly-6G/Ly-6C-BV605 (Biolegend, # 108440, 1:200); c-Kit-APCeFluor780 (eBioscience, #47-1171-82, 1:200); Sca-1-BV711 (Thermo Scientific, #11-5981-82, 1:200); CD48-AlexaFluor 700 (Biolegend, #103426, 1:200); CD150-BV785 Biolegend, # 115937, 1:200); CD34-PE (BD Biosciences #551387, 1:50); CD34-FITC (BD Biosciences #553733, 1:50); CD16/32-PerCP-Cy5.5 (Thermo Scientific, #45-0161, 1:100); CD71-PE ( eBioscience, #12-0711-83, 1:400); CD44-APC (Biolegend, #100412, 1:200); Ter119-FITC (BD Biosciences, #557915, 1:200); CD45R-APC-Cy7 (BD Biosciences, #552094, 1:200); CD11bAPC-Cy7 (BD Bioscience, #557657, 1:200); Ly6G/Ly6C-APC-Cy7 (BD Biosciences, #557661, 1:200); Annexin V-FITC (Biolegend, #640945, 1:200); GAPDH-HRP (Cell Signaling Technologies, #8884, 1:2,000); p53 (Cell Signaling Technologies, #2524, 1:1,000); Rps19 (Bethyl Labs, #A304-002A, 1:4,000); Rpl5 (Abcam, # ab86863, 1:4,000); AML1 (Cell Signaling Technologies, #4334, 1:10); RPS6 (Cell Signaling Technologies, #2217, 1:4,000); p-RPS6 (Cell Signaling Technologies, #4858, 1:2,000); 4EBP1 (Cell Signaling Technologies, # 9644, 1:1,000); p-4EBP1 (Cell Signaling Technologies, #2855, 1:1,000); p21 (BD Biosciences, #556431, 1:1,000); eEF2 (Cell Signaling Technologies, #2332, 1:1,000); p-eEF2 (Cell Signaling Technologies, #2331, 1:1,000); eIF2α (Cell Signaling Technologies, #5324, 1:4,000); p-eIF2α (Cell Signaling Technologies, #3398, 1:4,000); Actin-β (Cell Signaling Technologies, #4970, 1:4,000); eIF5a (BD Biosciences, #611976, 1:4,000); Hypusine (Creative Biolab, #PABL-202, 1:4,000); PABP1 (Cell Signaling Technologies, #4992, 1:1,000); eIF4E (Cell Signaling Technologies, #2067, 1:1,000); eIF4G (Cell Signaling Technologies, #2469, 1:1,000); eIF4H (Cell Signaling Technologies, # 3469, 1:1,000); AML (Cell Signaling Technologies, #8529, 1:1,000). .. Chemicals, peptides, and recombinant proteins Fetal bovine serum (Cytiva, #SH30071.03); IMDM (Fisher Scientific, #12-440-079); BSA (Fisher Scientific, #BP9703100); PBS (Cytiva, #SH30256.LS); RIPA buffer (Thermo Fisher Scientific, #89900); Agarose (IBI Scientific, #IB70042); Precision Plus Protein Dual Color Standards (BioRad, #1610374); 100 bp DNA Ladder (NEB, #N3231L); Proteinase K (Lamda Biotech, #DB0451-10); O-Propargyl-Puromycin (MedChem Express, #HY-15680); Sucrose (SigmaAldrich, #S9378-1KG); RiboSafe RNase Inhibitor (Bioline, #BIO-65028); Cycloheximide (SigmaAldrich, #C1988-1G); Sodium Chloride (Alfa Aesar, #12314-A3); Magnesium Chloride (SigmaAldrich, #M1028-100ML); DAPI (Sigma-Aldrich, #D9542-10mg); MethoCult M3334 (Stemcell Tech, #M3334); Methocult SF M3436 (Stemcell Tech, #M3436); MethoCult GF M3434 (Stemcell Tech, #M3434); CD117 Microbeads (Miltenyi Biotec, #130-091-224); Anti-Ter-119 MicroBeads (Miltenyi Biotec, #130-049-901); Phosphatase Inhibitor Cocktail (Bio Basic, #PL017); Protease Inhibitor Cocktail (Bimake, #B14002). .. Click-iT Plus EdU Assay Kit (Thermo Scientific, #C10632); Chromium Next GEM Single Cell 3’ Kit (10X Genomics, #1000268); Chromium Next GEM Single Cell Multiome ATAC+Gene Expression Reagent Bundle (10X Genomics, #1000283); Chromium Next GEM Chip G Single Cell Kit (10X Genomics, #1000120); Single Index Kit N, Set A (10X Genomics, #1000212); Dual Index Kit TT, Set A (10X Genomics, #1000215).

    Article Title: Neutrophil-Derived S100A8/A9 Drives Inflammation that Promotes Dnmt3a -Mutant Hematopoiesis
    Article Snippet: .. HSPCs (c-Kit + ) were isolated using CD117 MicroBeads (Miltenyi) and cultured in StemSpan SFEM II (StemCell Technologies) supplemented with 10 ng/mL recombinant murine SCF (StemCell Technologies) for 7 days at 37°C in 5% CO 2 . ..

    Protease Inhibitor:

    Article Title: RPS19 and RPL5 haploinsufficient models reveal divergent ribosomal subunit controls of fetal hematopoiesis.
    Article Snippet: The following antibodies were used: CD4-BV605 (Biolegend, #100451, 1:200); CD8a-BV6-5 (Biolegend, #100744, 1:200); Ter119-BV605 (Biolegend, #116239, 1:200); CD45R/B220-BV605 (Biolegend, #103224, 1:200); Ly-6G/Ly-6C-BV605 (Biolegend, # 108440, 1:200); c-Kit-APCeFluor780 (eBioscience, #47-1171-82, 1:200); Sca-1-BV711 (Thermo Scientific, #11-5981-82, 1:200); CD48-AlexaFluor 700 (Biolegend, #103426, 1:200); CD150-BV785 Biolegend, # 115937, 1:200); CD34-PE (BD Biosciences #551387, 1:50); CD34-FITC (BD Biosciences #553733, 1:50); CD16/32-PerCP-Cy5.5 (Thermo Scientific, #45-0161, 1:100); CD71-PE ( eBioscience, #12-0711-83, 1:400); CD44-APC (Biolegend, #100412, 1:200); Ter119-FITC (BD Biosciences, #557915, 1:200); CD45R-APC-Cy7 (BD Biosciences, #552094, 1:200); CD11bAPC-Cy7 (BD Bioscience, #557657, 1:200); Ly6G/Ly6C-APC-Cy7 (BD Biosciences, #557661, 1:200); Annexin V-FITC (Biolegend, #640945, 1:200); GAPDH-HRP (Cell Signaling Technologies, #8884, 1:2,000); p53 (Cell Signaling Technologies, #2524, 1:1,000); Rps19 (Bethyl Labs, #A304-002A, 1:4,000); Rpl5 (Abcam, # ab86863, 1:4,000); AML1 (Cell Signaling Technologies, #4334, 1:10); RPS6 (Cell Signaling Technologies, #2217, 1:4,000); p-RPS6 (Cell Signaling Technologies, #4858, 1:2,000); 4EBP1 (Cell Signaling Technologies, # 9644, 1:1,000); p-4EBP1 (Cell Signaling Technologies, #2855, 1:1,000); p21 (BD Biosciences, #556431, 1:1,000); eEF2 (Cell Signaling Technologies, #2332, 1:1,000); p-eEF2 (Cell Signaling Technologies, #2331, 1:1,000); eIF2α (Cell Signaling Technologies, #5324, 1:4,000); p-eIF2α (Cell Signaling Technologies, #3398, 1:4,000); Actin-β (Cell Signaling Technologies, #4970, 1:4,000); eIF5a (BD Biosciences, #611976, 1:4,000); Hypusine (Creative Biolab, #PABL-202, 1:4,000); PABP1 (Cell Signaling Technologies, #4992, 1:1,000); eIF4E (Cell Signaling Technologies, #2067, 1:1,000); eIF4G (Cell Signaling Technologies, #2469, 1:1,000); eIF4H (Cell Signaling Technologies, # 3469, 1:1,000); AML (Cell Signaling Technologies, #8529, 1:1,000). .. Chemicals, peptides, and recombinant proteins Fetal bovine serum (Cytiva, #SH30071.03); IMDM (Fisher Scientific, #12-440-079); BSA (Fisher Scientific, #BP9703100); PBS (Cytiva, #SH30256.LS); RIPA buffer (Thermo Fisher Scientific, #89900); Agarose (IBI Scientific, #IB70042); Precision Plus Protein Dual Color Standards (BioRad, #1610374); 100 bp DNA Ladder (NEB, #N3231L); Proteinase K (Lamda Biotech, #DB0451-10); O-Propargyl-Puromycin (MedChem Express, #HY-15680); Sucrose (SigmaAldrich, #S9378-1KG); RiboSafe RNase Inhibitor (Bioline, #BIO-65028); Cycloheximide (SigmaAldrich, #C1988-1G); Sodium Chloride (Alfa Aesar, #12314-A3); Magnesium Chloride (SigmaAldrich, #M1028-100ML); DAPI (Sigma-Aldrich, #D9542-10mg); MethoCult M3334 (Stemcell Tech, #M3334); Methocult SF M3436 (Stemcell Tech, #M3436); MethoCult GF M3434 (Stemcell Tech, #M3434); CD117 Microbeads (Miltenyi Biotec, #130-091-224); Anti-Ter-119 MicroBeads (Miltenyi Biotec, #130-049-901); Phosphatase Inhibitor Cocktail (Bio Basic, #PL017); Protease Inhibitor Cocktail (Bimake, #B14002). .. Click-iT Plus EdU Assay Kit (Thermo Scientific, #C10632); Chromium Next GEM Single Cell 3’ Kit (10X Genomics, #1000268); Chromium Next GEM Single Cell Multiome ATAC+Gene Expression Reagent Bundle (10X Genomics, #1000283); Chromium Next GEM Chip G Single Cell Kit (10X Genomics, #1000120); Single Index Kit N, Set A (10X Genomics, #1000212); Dual Index Kit TT, Set A (10X Genomics, #1000215).

    Isolation:

    Article Title: Stem cell function in vivo is supported by an alternative glycolysis endpoint
    Article Snippet: Flow cytometry antibodies were acquired from BD Biosciences, eBiosciences, BioLegend, or Tonbo biosciences. .. For isolation of HSPCs, cells were incubated with paramagnetic CD117 microbeads and enriched with a QuadroMACS magnetic separator (Miltenyi Biotec) before cell sorting. .. Analysis and cell sorting were performed using BD LSRFortessa (BD Biosciences), BD FACSCanto (BD Biosciences) or BD FACSSymphony S6 (BD Biosciences).

    Article Title: Sequential in vivo CRISPR screens identify the clonal dominance of Nf1 loss in long-term hematopoiesis
    Article Snippet: .. Primary Cas9-expressing HSPCs (3–6 × 10 6 cells) were isolated from individual Cas9 transgenic mice using either a Lineage Cell Depletion Kit (Miltenyi, 130-110-470, Bergisch Gladbach, Germany) or CD117 MicroBeads (Miltenyi, 130-097-146), according to the manufacturer’s instructions. ..

    Article Title: The TET-dependent DNA demethylation pathway is the driving force of hematopoiesis
    Article Snippet: To isolate mouse monocytes and granulocytes from bone marrow, we used CD11b MicroBeads UltraPure (130-126-725, Miltenyi Biotec, Auburn, CA USA). .. Cd117 (cKit) positive mouse cells were isolated from bone marrow using CD117 MicroBeads according to the MACS protocol (130-091-224, Miltenyi Biotec, Auburn, CA USA). ..

    Article Title: Neutrophil-Derived S100A8/A9 Drives Inflammation that Promotes Dnmt3a -Mutant Hematopoiesis
    Article Snippet: .. HSPCs (c-Kit + ) were isolated using CD117 MicroBeads (Miltenyi) and cultured in StemSpan SFEM II (StemCell Technologies) supplemented with 10 ng/mL recombinant murine SCF (StemCell Technologies) for 7 days at 37°C in 5% CO 2 . ..

    Incubation:

    Article Title: Stem cell function in vivo is supported by an alternative glycolysis endpoint
    Article Snippet: Flow cytometry antibodies were acquired from BD Biosciences, eBiosciences, BioLegend, or Tonbo biosciences. .. For isolation of HSPCs, cells were incubated with paramagnetic CD117 microbeads and enriched with a QuadroMACS magnetic separator (Miltenyi Biotec) before cell sorting. .. Analysis and cell sorting were performed using BD LSRFortessa (BD Biosciences), BD FACSCanto (BD Biosciences) or BD FACSSymphony S6 (BD Biosciences).

    FACS:

    Article Title: Stem cell function in vivo is supported by an alternative glycolysis endpoint
    Article Snippet: Flow cytometry antibodies were acquired from BD Biosciences, eBiosciences, BioLegend, or Tonbo biosciences. .. For isolation of HSPCs, cells were incubated with paramagnetic CD117 microbeads and enriched with a QuadroMACS magnetic separator (Miltenyi Biotec) before cell sorting. .. Analysis and cell sorting were performed using BD LSRFortessa (BD Biosciences), BD FACSCanto (BD Biosciences) or BD FACSSymphony S6 (BD Biosciences).

    Cell Characterization:

    Article Title: P300/CBP inhibition with inobrodib in combination with gilteritinib and venetoclax targets leukemia stem cells in epigenetic mutant AML.
    Article Snippet: Data analyses were performed using FlowJo software. .. Total cell counts for selected cell populations were calculated as follows: (gated population count/gated live- singlet count)*total cell count of mouse bone marrow or spleen. c- Kit+ enrichments of bone marrow or spleen AML cells were performed using CD117 microbeads according to the manufacturer’s instructions (Miltenyi Biotec) using AutoMACS Pro separator or magnetic stand (Miltenyi Biotec). ..

    Article Title: P300/CBP inhibition with inobrodib in combination with gilteritinib and venetoclax targets leukemia stem cells in epigenetic mutant AML
    Article Snippet: Data analyses were performed using FlowJo software. .. Total cell counts for selected cell populations were calculated as follows: (gated population count/gated live-singlet count)*total cell count of mouse bone marrow or spleen. c-Kit + enrichments of bone marrow or spleen AML cells were performed using CD117 microbeads according to the manufacturer’s instructions (Miltenyi Biotec) using AutoMACS Pro separator or magnetic stand (Miltenyi Biotec). ..

    Transgenic Assay:

    Article Title: Sequential in vivo CRISPR screens identify the clonal dominance of Nf1 loss in long-term hematopoiesis
    Article Snippet: .. Primary Cas9-expressing HSPCs (3–6 × 10 6 cells) were isolated from individual Cas9 transgenic mice using either a Lineage Cell Depletion Kit (Miltenyi, 130-110-470, Bergisch Gladbach, Germany) or CD117 MicroBeads (Miltenyi, 130-097-146), according to the manufacturer’s instructions. ..

    Magnetic Cell Separation:

    Article Title: The TET-dependent DNA demethylation pathway is the driving force of hematopoiesis
    Article Snippet: To isolate mouse monocytes and granulocytes from bone marrow, we used CD11b MicroBeads UltraPure (130-126-725, Miltenyi Biotec, Auburn, CA USA). .. Cd117 (cKit) positive mouse cells were isolated from bone marrow using CD117 MicroBeads according to the MACS protocol (130-091-224, Miltenyi Biotec, Auburn, CA USA). ..

    Cell Culture:

    Article Title: Neutrophil-Derived S100A8/A9 Drives Inflammation that Promotes Dnmt3a -Mutant Hematopoiesis
    Article Snippet: .. HSPCs (c-Kit + ) were isolated using CD117 MicroBeads (Miltenyi) and cultured in StemSpan SFEM II (StemCell Technologies) supplemented with 10 ng/mL recombinant murine SCF (StemCell Technologies) for 7 days at 37°C in 5% CO 2 . ..



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    The development of T cells is controlled by the TET dependent DNA demethylation pathway. A) Inactivation of TET enzymes results in the maintenance of high levels of methylation of genes essential for T cell development, according to DMRseq analysis (T_cells_KO - T cells isolated from the spleen of mice treated with tamoxifen; T_cells_C - T cells isolated from the spleen of control mice). B) PCA (B’), heatmap (B’’), and volcano plot (B’’’) indicate a significant difference in gene expression between TET-deficient (T_cell_KO) and control (T_cell_C) T cells. C) The heatmap shows that maintaining high levels of T cell gene methylation leads to a decrease in their expression. D) Many genes whose expression is reduced in TET-deficient T cells are necessary for their specification and maturation/function. At the same time, the expression of genes that are markers of HSPCs (Cd34, <t>Cd117)</t> is increased in TET-deficient T cells. Taken together, these results indicate the underdevelopment of these cells. CLP – common lymphoid progenitors, DN – double negative, DP – double positive
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    The development of T cells is controlled by the TET dependent DNA demethylation pathway. A) Inactivation of TET enzymes results in the maintenance of high levels of methylation of genes essential for T cell development, according to DMRseq analysis (T_cells_KO - T cells isolated from the spleen of mice treated with tamoxifen; T_cells_C - T cells isolated from the spleen of control mice). B) PCA (B’), heatmap (B’’), and volcano plot (B’’’) indicate a significant difference in gene expression between TET-deficient (T_cell_KO) and control (T_cell_C) T cells. C) The heatmap shows that maintaining high levels of T cell gene methylation leads to a decrease in their expression. D) Many genes whose expression is reduced in TET-deficient T cells are necessary for their specification and maturation/function. At the same time, the expression of genes that are markers of HSPCs (Cd34, Cd117) is increased in TET-deficient T cells. Taken together, these results indicate the underdevelopment of these cells. CLP – common lymphoid progenitors, DN – double negative, DP – double positive

    Journal: bioRxiv

    Article Title: The TET-dependent DNA demethylation pathway is the driving force of hematopoiesis

    doi: 10.64898/2026.04.29.721744

    Figure Lengend Snippet: The development of T cells is controlled by the TET dependent DNA demethylation pathway. A) Inactivation of TET enzymes results in the maintenance of high levels of methylation of genes essential for T cell development, according to DMRseq analysis (T_cells_KO - T cells isolated from the spleen of mice treated with tamoxifen; T_cells_C - T cells isolated from the spleen of control mice). B) PCA (B’), heatmap (B’’), and volcano plot (B’’’) indicate a significant difference in gene expression between TET-deficient (T_cell_KO) and control (T_cell_C) T cells. C) The heatmap shows that maintaining high levels of T cell gene methylation leads to a decrease in their expression. D) Many genes whose expression is reduced in TET-deficient T cells are necessary for their specification and maturation/function. At the same time, the expression of genes that are markers of HSPCs (Cd34, Cd117) is increased in TET-deficient T cells. Taken together, these results indicate the underdevelopment of these cells. CLP – common lymphoid progenitors, DN – double negative, DP – double positive

    Article Snippet: Cd117 (cKit) positive mouse cells were isolated from bone marrow using CD117 MicroBeads according to the MACS protocol (130-091-224, Miltenyi Biotec, Auburn, CA USA).

    Techniques: Methylation, Isolation, Control, Gene Expression, Expressing

    Demethylation and activation of many hematopoietic genes are suppressed early in hematopoiesis in TET-deficient experimental mice. A) The TET dependent DNA demethylation pathway initiates the demethylation of hematopoietic genes as early as the myeloid/lymphoid progenitor stage. DMRs were identified by comparing the methylomes of experimental (Cd117_KO) and control (Cd117_C) Cd117 + cells using the DMRseq Bioconductor package. B) Changes in methylation patterns caused by inactivation of TET enzymes lead to significant changes in gene expression in Cd117 + cells as evidenced by PCA (B’), heatmap (B’’), and volcano plot (B’’’). C) The heatmap reflects changes in the expression of many genes involved in hematopoiesis in Cd117_KO vs Cd117_C cells. D) The panel shows the stages of hematopoiesis and changes in the expression of genes corresponding to these stages. A green downward arrow indicates decreased gene expression in TET-deficient Cd117 + cells, while a red upward arrow indicates increased gene expression. E) To examine the rate of Cd117 + cell proliferation, EdU was administered intraperitonially into experimental and control mice. The bone marrow of these animals was collected after 30 minutes and used for Cd117 + cell isolation. Cd117 + cells were labeled, and the percentage (%) of cells incorporating EdU was determined using flow cytometry (n=5, ****P-value < 0.0001). F) The percentage (%) of apoptotic (Annexin V+/PI-; Q4) and necrotic (PI+; Q1 and Q2) Cd117 + cells was determined using flow cytometry (n=5, ****P-value < 0.0001, *P-value < 0.05).

    Journal: bioRxiv

    Article Title: The TET-dependent DNA demethylation pathway is the driving force of hematopoiesis

    doi: 10.64898/2026.04.29.721744

    Figure Lengend Snippet: Demethylation and activation of many hematopoietic genes are suppressed early in hematopoiesis in TET-deficient experimental mice. A) The TET dependent DNA demethylation pathway initiates the demethylation of hematopoietic genes as early as the myeloid/lymphoid progenitor stage. DMRs were identified by comparing the methylomes of experimental (Cd117_KO) and control (Cd117_C) Cd117 + cells using the DMRseq Bioconductor package. B) Changes in methylation patterns caused by inactivation of TET enzymes lead to significant changes in gene expression in Cd117 + cells as evidenced by PCA (B’), heatmap (B’’), and volcano plot (B’’’). C) The heatmap reflects changes in the expression of many genes involved in hematopoiesis in Cd117_KO vs Cd117_C cells. D) The panel shows the stages of hematopoiesis and changes in the expression of genes corresponding to these stages. A green downward arrow indicates decreased gene expression in TET-deficient Cd117 + cells, while a red upward arrow indicates increased gene expression. E) To examine the rate of Cd117 + cell proliferation, EdU was administered intraperitonially into experimental and control mice. The bone marrow of these animals was collected after 30 minutes and used for Cd117 + cell isolation. Cd117 + cells were labeled, and the percentage (%) of cells incorporating EdU was determined using flow cytometry (n=5, ****P-value < 0.0001). F) The percentage (%) of apoptotic (Annexin V+/PI-; Q4) and necrotic (PI+; Q1 and Q2) Cd117 + cells was determined using flow cytometry (n=5, ****P-value < 0.0001, *P-value < 0.05).

    Article Snippet: Cd117 (cKit) positive mouse cells were isolated from bone marrow using CD117 MicroBeads according to the MACS protocol (130-091-224, Miltenyi Biotec, Auburn, CA USA).

    Techniques: Activation Assay, Control, Methylation, Gene Expression, Expressing, Cell Isolation, Labeling, Flow Cytometry